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The block of the expressed L‐type calcium channel is modulated by the β 3 subunit
Author(s) -
Lacinová L.,
Ludwig A.,
Bosse E.,
Flockerzi V.,
Hofmann F.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01013-5
Subject(s) - calcium channel , isradipine , protein subunit , r type calcium channel , mibefradil , l type calcium channel , chemistry , verapamil , chinese hamster ovary cell , beta (programming language) , voltage dependent calcium channel , biophysics , endocrinology , n type calcium channel , alpha (finance) , medicine , calcium , t type calcium channel , dihydropyridine , biology , biochemistry , receptor , gene , construct validity , nursing , computer science , programming language , patient satisfaction
The α 1C subunit of the L‐type calcium channel was stable, expressed alone or in combination with the β 3 subunit in Chinese hamster ovary cells. The β 3 subunit enhanced significantly the inactivation of barium currents indicating that both subunits interacted with each other. The β 3 subunit decreased signinicantly the half‐maximal inhibitory concentration of the calcium channel blockers (−)‐gallopamil and verapamil, but did not affect significantly the block caused by isradipine and mibefradil at the holding potentials of −80 mV and −40 mV. These results suggest that the β 3 subunit affects distinctly the interaction of the expressed α 1c subunit with different classes of organic calcium channel blockers.