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Crystals of soluble interleukin‐1 receptor complexed with its natural antagonist reveal a 1:1 receptor‐ligand complex
Author(s) -
Schreuder Herman,
Tardif Chantal,
Soffientini Adolfo,
Sarubbi Edoardo,
Akeson Ann,
Bowlin Terry,
Yanofsky Stephen,
Barrett Ronald W.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01009-4
Subject(s) - interleukin 1 receptor antagonist , receptor antagonist , antagonist , competitive antagonist , interleukin 4 receptor , interleukin 21 receptor , receptor , interleukin 1 receptor, type ii , interleukin 1 receptor, type i , chemistry , recombinant dna , interleukin 13 receptor , ligand (biochemistry) , interleukin 1 receptor , stereochemistry , interleukin , biochemistry , cytokine , biology , immunology , insulin like growth factor 1 receptor , interleukin 5 , growth factor , gene
Interleukin‐1 is a cytokine involved in the acute phase response against infection and injury. We obtained crystals of a complex of soluble, recombinant human interleukin‐1 receptor and recombinant human interleukin‐1 receptor antagonist, a naturally occurring antagonist. The crystals are suitable for X‐ray analysis and diffract to 2.7 Å resolution. Solvent content calculations indicate that the crystals contain one receptor and one antagonist molecule per asymmetric unit. Other receptor to antagonist ratios are highly unlikely. These results suggest that the interleukin‐1 antagonist binds a single receptor molecule and does not cause receptor aggregation.