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Human pancreatic phospholipase A 2 stimulates the growth of human pancreatic cancer cell line
Author(s) -
Hanada Keiji,
Kinoshita Eiji,
Itoh Masaki,
Hirata Manabu,
Kajiyama Goro,
Sugiyama Masanori
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01005-y
Subject(s) - pancreatic cancer , pancreas , enzyme , cell culture , phospholipase , scatchard plot , phospholipase a , phospholipase a2 , cell growth , growth inhibition , chemistry , biology , biochemistry , binding site , endocrinology , medicine , cancer , genetics
Phospholipase A 2 (PLA 2 ) from human pancreas, designated hPLA 2 ‐I, functions as a digestive enzyme. Interestingly, the present study demonstrated that the mature form of hPLA 2 ‐I stimulated the growth of a human pancreatic cancer cell line MIAPaCa‐2, whereas the pro‐form was ineffective. PLA 2 s from Laticauda semifasciata fraction I, Crotalus adamanteus venom, Streptomyces violaceoruber and bee venom, showed no proliferative effect to the growth of MIAPaCa‐2. The Scatchard plot analysis revealed that the MIAPaCa‐2 cell had a specific binding site for the mature hPLA 2 ‐I. The equilibrium binding constant ( K d ) and the maximum binding capacity ( B max ) were 2.6 nM and 0.4 fmol/10 6 cells, respectively. These results suggest that the mature hPLA 2 ‐I, but not the pro‐form, may function as a growth factor of pancreas carcinoma via the specific binding site.