Premium
Involvement of protein kinase in Δ 12 ‐prostaglandin J 2 ‐induced expression of rat heme oxygenase‐1 gene
Author(s) -
Negishi Manabu,
Odani Noriko,
Koizumi Tomonobu,
Takahashi Senye,
Ichikawa Atsushi
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01001-u
Subject(s) - heme oxygenase , nuclear protein , gene expression , protein kinase a , microbiology and biotechnology , gene , staurosporine , heme , messenger rna , chemistry , kinase , biology , biochemistry , enzyme , transcription factor
We recently identified the cis ‐regulatory element and its specific nuclear binding factors for Δ 12 ‐prostaglandin (PG) J 2 ‐induced expression of the rat heme oxygenase, HO‐1 [Koizumi, T., Odani, N., Okuyama, T., Ichikawa, A. and Negishi, M. (1995)J. Biol. Chem. 270, in press]. Here we further examined the molecular mechanism underlying the Δ 12 ‐PGJ 2 ‐induced HO‐1 gene expression. Protein kinase inhibitors, 2‐aminopurine and staurosporine, suppressed the Δ 12 ‐PGJ 2 ‐induced HO‐1 mRNA and the nuclear protein binding to the Δ 12 ‐PGJ 2 ‐responsive cis ‐regulatory element in rat basophilic leukemia cells. Furthermore, the nuclear protein binding to the element was suppressed by in vitro phosphatase treatment of the nuclear proteins from Δ 12 ‐PGJ 2 ‐treated cells. These findings suggest that Δ 12 ‐PGJ 2 induces the expression of the HO‐1 gene through phosphorylation of the nuclear proteins which bind to the Δ 12 ‐PGJ 2 ‐responsive element.