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Localisation of metallothionein isoform mRNAs in rat hepatoma (H4) cells
Author(s) -
Mahon Patrick,
Beattie John,
Glover L.Anne,
Hesketh John
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01000-5
Subject(s) - polysome , metallothionein , gene isoform , cytoskeleton , messenger rna , microbiology and biotechnology , rna , biology , oligonucleotide , translation (biology) , biochemistry , chemistry , gene , cell , ribosome
The localisation of metallothionein isoform mRNAs in rat hepatoma (H4) cells was investigated using two approaches, namely Northern hybridisation of total RNA extracted from free, cytoskeletal‐bound and membrane‐bound polysomes isolated by a sequential detergent/salt extraction procedure and in situ hybridisation. The cytoskeletal‐bound polysomes were enriched in metallothionein‐I (MT‐I) and c‐myc mRNAs but showed a significantly lower enrichment in MT‐II mRNA. These findings indicate that the MT‐I mRNA is localised to the cytoskeleton during translation. In situ hybridisation using a biotin‐labelled oligonucleotide probe revealed a predominantly perinuclear localisation for the MT‐I mRNA.