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Induction of apoptotic cell death by pancreatitis‐associated ascitic fluid in Madin‐Darby canine kidney cells
Author(s) -
Nishikawa Junsuke,
Takeyama Yoshifumi,
Ueda Takashi,
Hori Yuichi,
Ueno Nozomi,
Yamamoto Masahiro,
Saitoh Yoichi
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00999-p
Subject(s) - ascitic fluid , apoptosis , programmed cell death , pancreatitis , kidney , medicine , apoptotic cell death , chemistry , biochemistry , ascites
We investigated the cytotoxicity on Madin‐Darby canine kidney (MDCK) cells of pancreatitis‐associated ascitic fluid (PAAF) collected from rats with experimental necrotizing pancreatitis. PAAF reduced viability of MDCK cells in a time‐ and dose‐dependent manner. We detected DNA fragmentation on the PAAF‐treated MDCK cells, indicating that the cytocidal action of PAAF is via apoptosis. From the results obtained, we conclude that PAAF contains factor(s) inducing apoptosis on MDCK cells, and we assume that apoptotic cell death is involved in the mechanism of organ failure in acute pancreatitis.