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NOR‐2 (neuron‐derived orphan receptor), a brain zinc finger protein, is highly induced during liver regeneration
Author(s) -
Petropoulos Isabelle,
Part Dominique,
Ochoa Alberto,
Zakin Mario M.,
Lamas Eugénia
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00998-o
Subject(s) - zinc finger , cycloheximide , biology , microbiology and biotechnology , complementary dna , nuclear receptor , protein biosynthesis , messenger rna , receptor , biochemistry , transcription factor , gene
Zinc‐finger proteins are involved in several cellular processes. Some of these proteins are implicated in the primary cellular response in regenerating liver and mitogen‐stimulated cells. Using a rat cDNA brain library, we have isolated a clone designated NOR‐2, encoding a protein containing two zinc‐finger motifs and whose expression is highly induced during G0/G1 transition. We analysed the expression of NOR‐2 mRNAs during early growth in regenerating liver and in both insulin‐stimulated H4‐II cells and pheochromocytoma‐derived cell line PC12 treated by NGF. In these systems, there is an early, rapid and transient accumulation of NOR‐2 mRNAs. The induction of NOR‐2 mRNAs does not require de novo protein synthesis, since it is not prevented by cycloheximide treatment. Mobility shift assays show that NOR‐2 protein binds to NBRE, a target sequence for r‐NGFI‐B family. Structurally, NOR‐2 is closely related to the recently identified NOR‐1 factor. Therefore, like NOR‐1, NOR‐2 belongs to the r‐NGFI‐B sub‐family of nuclear receptors superfamily.