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Transforming growth factor‐ β 1 downregulates dexamethasone‐indunced tetranectin gene expression during the in vitro mineralization of the human osteoblastic cell line SV‐HFO
Author(s) -
Iba Kousuke,
Sawada Norimasa,
Chiba Hideki,
Wewer Ulla M.,
Ishii Seiichi,
Mori Michio
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00992-i
Subject(s) - alkaline phosphatase , dexamethasone , cell culture , gene expression , mineralization (soil science) , in vitro , chemistry , transforming growth factor , northern blot , microbiology and biotechnology , biology , endocrinology , gene , biochemistry , enzyme , genetics , organic chemistry , nitrogen
In the present study, we examined the regulation of tetranectin gene expression using a human osteoblastic cell line, SV‐HFO, that undergoes mineralization upon treatment with dexamethasone. We found that the expression of tetranectin and alkaline phosphatase mRNA was induced by dexamethasone treatment as evidenced by Northern blotting. When transforming growth factor‐ β 1 (TGF‐ β 1 ) was added together wwith dexamethasone to the SV‐HFO cell cultures, the mineralization process was markedly suppressed and the expression of tetra nectin and alkaline phosphatase was downregulated in a dosedependent manner. These results demonstrate that the expression of tetranectin in these osteoblastic cells is regulated by dexamethasone and TGF‐ β 1 and that tetranectin expression is tightly linked to the process of mineralization.