Premium
Cross‐linking of at least three binding sites mediates signal transduction in a CR2‐positive Burkitt lymphoma derived cell line (Raji)
Author(s) -
Barile Guiseppe,
Pernozzoli Barbara,
Tiracchia Valentina,
Ioppolo Salvatore,
De Cristofaro Maria,
Mattei Elisabetta,
Faggioni Alberto
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00989-m
Subject(s) - raji cell , signal transduction , burkitt's lymphoma , microbiology and biotechnology , cell culture , polyclonal antibodies , cell , lymphoma , biology , chemistry , antibody , biochemistry , immunology , genetics
In this study we demonstrate that Raji cells, a CR2‐positive Burkitt lymphoma‐derived cell line, during cell growth, need the cross‐linking of multiple OKB7 binding sites or C3d determinants to mediate signal transduction. The loss of one of these affects the cellular response. Moreover, OKB7, the anti‐CR2 MoAb, recognizes C3d determinants on the cell surface and inhibits signal transduction induced by anti‐C3d polyclonal antibody. Since Raji cells are always CR2 positive during cell growth, we suppose that at least another protein, along with CR2, may be involved in setting up a cell surface complex able to receive and transduce the signal triggered by OKB7. In our experimental system the protein that offers a third binding site to OKB7, may be represented by a 33 kDa protein bearing C3d determinants.