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The mouse prostaglandin E receptor EP 2 subtype: cloning, expression, and Northern blot analysis
Author(s) -
Katsuyama Masato,
Nishigaki Nobuhiro,
Sugimoto Yukihiko,
Morimoto Kimiko,
Negishi Manabu,
Narumiya Shuh,
Ichikawa Atsushi
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00966-d
Subject(s) - chinese hamster ovary cell , northern blot , microbiology and biotechnology , agonist , biology , receptor , western blot , prostaglandin e2 receptor , complementary dna , clone (java method) , endocrinology , biochemistry , gene
A functional cDNA clone for the mouse prostaglandin (PG) E receptor EP 2 subtype was isolated from a mouse cDNA library. The mouse EP 2 receptor consists of 362 amino acid residues with seven putative transmembrane domains. [ 3 H]PGE 2 bound specifically to the membrane of Chinese hamster ovary cells stably expressing the cloned receptor. This binding was displaced by unlabeled prostanoids in the order of iloprost, a stable PGI 2 agonist > PGF 2 α > PGD 2 . Binding was also inhibited by butaprost (an EP 2 agonist) and to a lesser extent by M&B 28767 (an EP 3 agonist), but not by sulprostone (an EP 1 and EP 3 agonist) or SC‐19220 (an EP 1 antagonist). PGE 2 and butaprost increased the cAMP level in the Chinese hamster ovary cells in a concentration‐dependent manner. Northern blot analysis revealed that EP 2 mRNA is expressed most abundantly in the uterus, followed by the spleen, lung, thymus, ileum, liver, and stomach.