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Isolation of a novel ras ‐recision gene that is induced by hydrogen peroxide from a mouse osteoblastic cell line, MC3T3‐E1
Author(s) -
Egawa Kiyoshi,
Yoshiwara Mitsue,
Shibanuma Motoko,
Nose Kiyoshi
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00957-b
Subject(s) - complementary dna , microbiology and biotechnology , cell culture , messenger rna , epidermal growth factor , gene , biology , cdna library , gene expression , amino acid , chemistry , biochemistry , genetics
Hydrogen peroxide appears to mediate growth factor actions, and it inhibits DNA synthesis in normal mouse osteoblastic cells (MC3T3‐E1) at non‐toxic doses. However the sensitivity of cells to H 2 O 2 is greatly decreased in their ras ‐transformants. To understand the molecular basis of this sensitivity to H 2 O 2 , we attempted to identify H 2 O 2 ‐inducible cDNA clones from MC3T3 cells by differential screening of cDNA libraries, and one of such genes, named HIC‐53, was isolated. The level of HIC‐53 mRNA was moderately increased by H 2 O 2 as well as by calcium ionophore or dexamethasone, but was not increased by the addition of serum, tumor promotimg phorbol ester, or epidermal growth factor. Among mouse organs, HIC‐53 mRNA levels were higher in the kidney and lung, but were almost undetectable in the brain, heart, bone, muscle or spleen. In MC3T3 cells transformed with v‐Ki‐ ras , the HIC‐53 mRNA level was markedly decreased, and effect of H 2 O 2 was abolished. Although the biological function of HIC‐53 is unknown at present, the predicted amino acid sequence exhibited some similarity with bovine cardiac Na + /Ca + exchanger. The nucleotide sequence of HIC‐53 cDNA showed no significant similarity with other known gene sequences.