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The voltage‐dependent K + channel (Kv1.5) cloned from rabbit heart and facilitation of inactivation of the delayed rectifier current by the rat β subunit
Author(s) -
Sasaki Yoshiteru,
Ishii Kuniaki,
Nunoki Kazuo,
Yamagishi Toshio,
Taira Norio
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00954-8
Subject(s) - protein subunit , chemistry , rabbit (cipher) , biophysics , facilitation , channel (broadcasting) , rectifier (neural networks) , microbiology and biotechnology , biology , biochemistry , electrical engineering , neuroscience , gene , computer science , engineering , stochastic neural network , computer security , machine learning , recurrent neural network , artificial neural network
We have isolated a cDNA coding for a delayed rectifier K + channel (RBKV1.5) from rabbit heart. The amino acid sequence of RBKV1.5 displays a homology to that of other K + channels of Kv1.5 class. Overall amino acid identity between RBKV1.5 channel and Kv1.5 channel of other species is about 85%. RNA blot analysis revealed the expression of the primary transcript in various rabbit tissues, at the highest level in both the atrium and ventricle. When expressed in Xenopus oocytes , RBKV1.5 current showed a delayed rectifier type characteristics, which was converted to rapidly inactivating currents upon coexpression with a β subunit.