Premium
Concomitant expression of hepatocyte growth factor (HGF), HGF activator and c‐met genes in human glioma cells in vitro
Author(s) -
Moriyama Takuzou,
Kataoka Hiroaki,
Tsubouchi Hirohito,
Koono Masashi
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00949-a
Subject(s) - hepatocyte growth factor , activator (genetics) , glioma , autocrine signalling , c met , in vitro , microbiology and biotechnology , biology , cell culture , cancer research , paracrine signalling , chemistry , gene , receptor , biochemistry , genetics
Three new cell lines of human glioblastoma have been established. These cells co‐expressed hepatocyte growth factor (HGF) and its receptor, c‐Met, genes in vitro. Reverse‐transcriptase/polymerase‐chain reaction study revealed that the cells also expressed gene for HGF activator, a recently cloned serine proteinase, suggesting that HGF might have a role in glioma cells in vitro as an autocrine factor. The activator mRNA was also detected in other well‐established glioma cell lines, glioma tissues and normal brain. The concomitant expression of HGF, HGF activator and c‐met was also detected in one glioblastoma case in vivo out of five tested.