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Transfection of cells with basic fibroblast growth factor and Kaposi fibroblast growth factor genes induce resistance to and receptor modulation of tumor necrosis factor
Author(s) -
Aggarwal Bharat B.,
Pocsik Eva,
Totpal Klara
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00945-6
Subject(s) - transfection , tumor necrosis factor alpha , fibroblast growth factor receptor 3 , fibroblast growth factor receptor 2 , fibroblast growth factor , fibroblast growth factor receptor 4 , biology , basic fibroblast growth factor , microbiology and biotechnology , fgf1 , fibroblast growth factor receptor , fibroblast , growth factor , cancer research , receptor , cell culture , immunology , biochemistry , genetics
Tumor necrosis factor (TNF) has been shown to inhibit the growth of some cell types and stimulate the proliferation of others by a mechanism that is not understood. In the present study, we investigated the effect of transfection of NIH‐3T3 cells with either the basic fibroblast growth factor gene ( bFGF ) or the kaposi FGF gene ( K‐fgf ) on the growth‐modulatory effects of TNF. Our results show that transformation of cells with either gene leads to resistance to the growth‐inhibitory effects of TNF. The K‐fgf gene was found to be a more potent inducer of cellular resistance than the bFGF gene. The cellular resistance correlated with the inhibition of TNF‐induced activation of phospholipase A 2 and downmodulation of TNF receptors. Overall, our results indicate that both K‐fgf and bFGF play an important role in suppression of antiproliferative effects of TNF.