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Stable expression of maize auxin‐binding protein in insect cell lines
Author(s) -
Jeffrey Henderson,
Allan E. Atkinson,
Colin M. Lazarus,
Chris Hawes,
Richard Napier,
Heather Macdonald,
Linda A. King
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00922-v
Subject(s) - kdel , sf9 , signal peptide , endoplasmic reticulum , spodoptera , microbiology and biotechnology , biology , gene , transfection , gene expression , peptide sequence , biochemistry , recombinant dna , golgi apparatus
To achieve continuous expression of the major maize auxin‐binding protein (ABP1) in insect cells, the ABP1 gene coding region was placed under control of a baculovirus immediate‐early gene promoter and transfected into Spodoptera frugiperda Sf9 cells. The ABP1 gene was detected in twelve cell lines, one of which was selected for detailed analysis. Immunolocalisation demonstrated that ABP1 was targeted to and retained in the endoplasmic reticulum (ER), in accordance with its signal peptide and car☐y‐terminal KDEL ER‐retention signal. We discuss the advantages of stable‐transformation over transient expression systems for characterising proteins targeted to the secretory system of insect cells.