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Accumulation of cyclic ADP‐ribose measured by a specific radioimmunoassay in differentiated human leukemic HL‐60 cells with all‐ trans ‐retinoic acid
Author(s) -
Takahashi Katsunobu,
Kukimoto Iwao,
Tokita Ken-ichi,
Inageda Kiyoshi,
Inoue Shin-ichi,
Kontani Kenji,
Hoshino Shin-ichi,
Nishina Hiroshi,
Kanaho Yasunori,
Katada Toshiaki
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00914-u
Subject(s) - cd38 , cyclic adp ribose , radioimmunoassay , retinoic acid , nad+ kinase , intracellular , chemistry , cyclase , second messenger system , biochemistry , cell culture , adenosine , enzyme , microbiology and biotechnology , biology , stem cell , genetics , cd34 , gene
Cyclic adenosine diphosphoribose (cADPR) is a novel candidate for the mediator of Ca 2+ release from intracellular Ca 2+ stores. The formation of this cyclic nucleotide is catalyzed by not only Aplysia ADP‐ribosyl cyclase but also an ecto‐form enzyme of NAD + glycohydrolase (NADase), which was previously identified as all‐ trans ‐retinoic acid (RA)‐inducible CD38 in human leukemic HL‐60 cells. In the present study, we developed a radioimmunoassay specific for cADPR, by which more than 100 fmol of cADPR could be detected without any interference by other nucleotides. The possible involvement of CD38 in the formation of cellular cADPR was investigated with the radioimmunoassay method. A marked increase in cellular cADPR was accompanied by all‐ trans ‐RA‐induced differentiation of HL‐60 cells. Moreover, a high level of cellular cADPR was observed in other leukemic cell lines, in which CD38 mRNA was expressed. Thus, CD38, which was initially identified as an NADase, appeared to be responsible for the formation of cellular cADPR.