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Complete amino acid sequence of the protease inhibitor from buckwheat seeds
Author(s) -
M. A. Belozersky,
Yakov E. Dunaevsky,
Alexander Musolyamov,
Tsezi A. Egorov
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00899-k
Subject(s) - edman degradation , protease , protease inhibitor (pharmacology) , amino acid , peptide sequence , biochemistry , molecular mass , chemistry , sequence (biology) , fagopyrum , biology , enzyme , botany , gene , human immunodeficiency virus (hiv) , antiretroviral therapy , viral load , immunology
The complete amino acid sequence of protease inhibitor BWI‐1 from buckwheat ( Fagopyrum esculentum Moench) seeds has been established by automatic Edman degradation and mass spectrometry. The molecule of the inhibitor consists of 69 amino acid residues, with a molecular mass calculated as 7743.8 Da. The active site of the inhibitor containes an Arg 45 ‐Asp 46 bond. Analysis of the amino acid sequence suggests that the buckwheat seed protease inhibitor is a member of the proteinase inhibitor I family.