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Effect of cyclosporin A on energy coupling in pea stem mitochondria
Author(s) -
Angelo Vianello,
Francesco Macrı̀,
Enrico Braidot,
E. N. Mokhova
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00897-i
Subject(s) - oligomycin , rotenone , mitochondrion , mitochondrial permeability transition pore , biophysics , chemistry , biochemistry , biology , coupling (piping) , permeability (electromagnetism) , atpase , apoptosis , membrane , materials science , enzyme , programmed cell death , metallurgy
Effect of cyclosporin A on energy coupling in pea stem mitochondria is studied. It is found that incubation of mitochondria with 100 nM FCCP and/or CAtr, oligomycin, CaCl 2 , palmitate and ADP results, after some lag phase, in a collapse of Δ/ψ generated by succinate oxidation in the presence of rotenone. Cyclosporin A (0.2 – 0.8 nmol/mg mitochondrial protein) markedly increases the lag phase. The cyclosporin A effect requires dithioerythritol to be added to the isolation medium. Metabisulphite fails to substitute for dithioerythritol. The relationships between these effects and cyclosporin A‐sensitive mitochondrial permeability transition in animal mitochondria are discussed.