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ADP‐ribosylation of Rho enhances actin polymerization‐coupled shape oscillations in human neutrophils
Author(s) -
Ehrengruber Markus U.,
Boquet Patrice,
Coates Thomas D.,
Deranleau David A.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00880-i
Subject(s) - actin , motility , polymerization , biophysics , adp ribosylation , chemistry , microbiology and biotechnology , biology , biochemistry , polymer , enzyme , nad+ kinase , organic chemistry
Stimulated neutrophils exhibit coordinated sinusoidal oscillations in filamentous actin content and cellular shape. We investigated the effect of inhibition of the small G protein Rho on neutrophil actin polymerization, shape changes and oscillations using a genetically engineered toxin that enters cells and selectively ADP‐ribosylates endogenous Rho. This treatment increased the amplitudes and frequencies of shape oscillations and duration of the oscillating transient. However, it had no effect on the initial actin polymerization and shape changes induced by N ‐formyl‐Met‐Leu‐Phe. Regulation of these oscillations may be important for the control of neutrophil motility.