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Modulation of soybean lipoxygenase expression and membrane oxidation by water deficit
Author(s) -
Mauro Maccarrone,
Gerrit A. Veldink,
Alessandro Finazzi Agrò,
Johannes F.G. Vliegenthart
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00876-b
Subject(s) - lipoxygenase , abscisic acid , chemistry , gene expression , biochemistry , oxidative stress , oxidative phosphorylation , enzyme , microbiology and biotechnology , gene , biology
The modulation of the activity and expression of soybean lipoxygenases 1 (LOX‐1) [Vliegenthart, J.F.G. and Veldink, G.A. (1982) in: Free Radicals in Biology (Pryor, W.A., Ed.) pp. 29–64, Academic Press, New York] and 2 (LOX‐2) by water deficit (osmotic stress) has been investigated, by following gene expression at the transcriptional and translational levels. Osmotic stress enhanced the transcription of the genes of both isoenzymes, leading to increased levels of the corresponding mRNAs, protein contents and specific activities. Abscisic acid (ABA) did not mediate enhancement of LOX expression, but caused a decrease of LOX‐2 activity and was ineffective on LOX‐1. Water deficit also caused oxidative modification of soybean membrane pool lipids [Schmidt, W.E. and Ebel, J. (1987) Proc. Natl. Acad. Sci. USA 84, 4117–4121], attributable to the increase of conjugated hydroperoxides in the esterified fatty acids of the lipid bilayer.