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Abolition of G protein inhibition of α 1A and α 1B calcium channels by co‐expression of the β 3 subunit
Author(s) -
Roche John P.,
Anantharam Vellareddy,
Treistman Steven N.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00860-c
Subject(s) - protein subunit , chemistry , protein expression , calcium , biophysics , biochemistry , biology , gene , organic chemistry
Three different classes of α 1 Ca 2+ channel ( α 1A , α 1B , α 1C ) were expressed in Xenopus oocytes to determine whether G protein‐mediated inhibition is an inherent property of the α 1 , subunit itself, and if so, whether co‐expression of auxiliary subunits modulates the inhibition seen. From our data it is apparent that either α 1A or α 1B Ca 2+ channels expressed alone are sufficient for voltage‐dependent G protein inhibition. α 1C Ca 2+ channels expressed alone do not exhibit the G protein inhibition seen in α 1A and α 1B channels. Additionally, co‐expression of the β 3 subunit abolishes the ability of G proteins to inhibit currents through α 1A and α 1B Ca 2+ channels. Differential sensitivity of α 1 as well as modulation of properties by β 3 provide a potential mechanism for the regulation of G protein‐mediated inhibition in neurons.