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Microtubule associated protein 1B (MAP1B) promotes efficient tubulin polymerisation in vitro
Author(s) -
Pedrotti Barbara,
Islam Khalid
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00842-w
Subject(s) - tubulin , microtubule , in vitro , polymerization , kinetics , chemistry , microtubule associated protein , reaction rate constant , constant (computer programming) , biophysics , microbiology and biotechnology , biochemistry , biology , organic chemistry , computer science , physics , programming language , quantum mechanics , polymer
The effect of MAP1B on tubulin polymerisation has been examined in reconstitution experiments using purified tubulin and MAP1B. Under the assembly conditions used, tubulin alone was incapable of polymerising, but addition of MAP1B resulted in rapid assembly into microtubules. The kinetics of MAP1B‐promoted microtubule assembly examined using pseudo‐first‐order plots show that assembly is described by a single reaction rate. The calculated association rate constant for MAP1B was about 200 × 10 6 M −1 ·s −1 and this constant was one order of magnitude higher when compared with that for MAP2‐promoted assembly.