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Enhancement of aromatic amino acid‐nucleic acid base stacking interaction by metal coordination to base: fluorescence study on a tryptophan‐Pt(II)‐guanine ternary complex
Author(s) -
Hisahiro Kawai,
Mariko Tarui,
Mitsunobo Doi,
Toshimasa Ishida
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00824-s
Subject(s) - guanine , stacking , chemistry , tryptophan , nucleic acid , metal ions in aqueous solution , fluorescence , quenching (fluorescence) , metal , ternary complex , ion , inorganic chemistry , photochemistry , amino acid , nucleotide , biochemistry , organic chemistry , physics , quantum mechanics , gene , enzyme
In order to investigate the effect of the Pt(II) ion on the stacking interaction between tryptophan and a guanine base, the quenching of Trp fluorescence was monitored for some systems in the absence and presence of the metal ion, and the association constants were obtained by the analysis of Eadie‐Hofstee plots. All spectral data suggested that the stacking interaction is enhanced by the Pt(II) coordination to the guanine N7 atom. The result indicates the importance of the metal ion as a bookmark in the specific recognition of a nucleic acid base by an aromatic amino acid residue.