Premium
Secretion and processing mechanisms of procathepsin L in bone resorption
Author(s) -
Kakegawa Hisao,
Tagami Kahori,
Ohba Yasuo,
Sumitani Koji,
Kawata Terushige,
Katunumaa Nobuhiko
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00790-g
Subject(s) - secretion , bone resorption , chemistry , cathepsin , parathyroid hormone , resorption , cathepsin d , endocrinology , cathepsin k , enzyme , medicine , biochemistry , calcium , biology , in vitro , osteoclast , organic chemistry
Secretion of procathepsin L into the culture medium from a bone cell mixture was markedly enhanced by addition parathyroid hormone (PTH), 1α,25‐(OH) 2 D 3 or tumor necrosis factor α (TNF α ). These stimulators of secretion of procathepsin L enhanced bone pit formation, which was inhibited by E‐64, but not by CA‐074, a specific inhibitor of cathepsin B. Procathepsin L may thus participate in the process of bone collagenolysis during bone resorption. Procathepsin L partially purified from rat long bones under cold conditions was rapidly converted to the mature form under acidic conditions at room temperature. This conversion was inhibited by E‐64, suggesting that the procathepsin L secreted into lacunae is catalytically converted to the mature enzyme by cysteine proteinase(s).