Premium
Molecular cloning and sequence analysis of human preprocathepsin C
Author(s) -
Pariš Alenka,
Štrukelj Borut,
Pungerčar Jože,
Renko Metka,
Dolenc Iztok,
Turk Vito
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00777-7
Subject(s) - complementary dna , peptide sequence , microbiology and biotechnology , nucleic acid sequence , molecular cloning , biology , sequence analysis , coding region , amino acid , sequence (biology) , protein sequencing , sequence alignment , biochemistry , gene
A cDNA clone (C1) coding for human preprocathepsin C was isolated from a human ileum cDNA library using a rat kidney‐derived RT‐PCR probe and its complete nucleotide sequence determined. The full‐length 1857 bp sequence codes for a protein of 463 amino acid residues with a calculated molecular mass of 51848 Da. Comparison of the deduced amino acid sequence with that of rat preprocathepsin C indicates an 87.5% identity. A multiple alignment of the deduced cathepsin C sequence of 233 residues which, by analogy to other cysteine proteinases, corresponds to the mature protein, confirms that human cathepsin C belongs to the papain superfamily.