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Effects of vasoactive substances and cAMP related compounds on adrenomedullin production in cultured vascular smooth muscle cells
Author(s) -
Sugo Shin,
Minamino Naoto,
Shoji Hiroki,
Kangawa Kenji,
Matsuo Hisayuki
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00769-6
Subject(s) - adrenomedullin , forskolin , vascular smooth muscle , medicine , endocrinology , bradykinin , angiotensin ii , chemistry , receptor , intracellular , adenylate kinase , endothelin receptor , vasoactive , cyclase , biology , biochemistry , smooth muscle
To elucidate the regulation mechanism of adrenomedullin (AM) production in blood vessels, we examined the effects of 30 substances on AM production in cultured rat vascular smooth muscle cells (VSMCs). Forskolin and 8‐bromo‐cAMP suppressed production and gene transcription of AM. Since VSMC expresses AM receptors coupled with adenylate cyclase, AM production may be regulated by intracellular cAMP concentration. Thrombin, vasoactive intestinal polypeptide and interferon‐gg also inhibited AM production, while angiotensin II, endothelin‐1, bradykinin, substance P, adrenaline, phorbol ester and fetal calf serum stimulated AM production in VSMC. These results suggest that AM production is regulated by a variety of substances, indicating complex systems regulating AM production.