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Cloning and characterization of a cDNA encoding a cellobiose dehydrogenase from the white rot fungus Phanerochaete chrysosporium
Author(s) -
Raices Manuel,
Paifer Edenia,
Cremata José,
Montesino Raquel,
Ståhlberg Jerry,
Divne Christina,
Szabó István J,
Henriksson Gunnar,
Johansson Gunnar,
Pettersson Göran
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00758-2
Subject(s) - phanerochaete , chrysosporium , cellobiose dehydrogenase , cellobiose , cloning (programming) , fungus , complementary dna , white rot , biology , microbiology and biotechnology , botany , gene , biochemistry , enzyme , cellulase , lignin , computer science , programming language
The cDNA of cellobiose dehydrogenase (CDH) from Phanerochaete chrysosporium has been cloned and sequenced. The 5′ end was obtained by PCR amplification. The cDNA contains 2310 translated bases excluding the poly(A) tail. The deduced mature protein contains 770 amino acid residues and is preceded by a 18 residue long signal peptide. The regions of the amino acid sequence corresponding to the heme and FAD domains of CDH were identified as well as the nucleotide‐binding motif, the disulfide pairing and a methionine residue chelating the heme iron. No homologous sequences were found for the heme domain, however, the FAD domain appears to be distantly related to the GMC oxidoreductase family.