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Ribosomal protein L22 from Thermus thermophilus : sequencing overexpression and crystallisation
Author(s) -
Davydova Natalia L.,
Gryaznova Olga I.,
Mashchenko Oksana V.,
Vysotskaya Valentina S.,
Jonsson Bengt-Harald,
Al-Karadaghi Salam,
Liljas Anders,
Garber Maria B.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00755-x
Subject(s) - thermus thermophilus , ribosomal protein , escherichia coli , recombinant dna , gene , ribosomal rna , microbiology and biotechnology , ribosome , chemistry , biology , biochemistry , rna
The gene for the ribosomal protein L22 from Thermus thermophilus has been sequenced and overexpressed in Escherichia coli . A multiple sequence alignment was carried out for all proteins of the L22 family reported so far. The recombinant protein was purified and crystallized. The crystals belong to the space group P 2 1 2 1 2 1 with cell parameters of .