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Interaction of rat liver lysosomes with basic polypeptides
Author(s) -
Rukavishnikova Elena V.,
Korolenko Tatjana A.,
Sassa Toshihiro,
Oka Tatsuzo,
Horiuchi Saburou,
Natoria Yasuo
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00749-y
Subject(s) - polyglutamic acid , polylysine , biochemistry , lysosome , incubation , chemistry , enzyme , cytosol , in vitro , microbiology and biotechnology , biology
In order to gain knowledge on the interaction of lysosomes with proteins, we have assessed the equilibrium densities of the lysosomal membrane and matrix markers after in vitro incubation of rat liver lysosomes with various polypeptides. The addition of basic polypeptides, polylysine or protamine, to the suspension of lysosomes brought about a profound alteration of lysosomal membrane, causing extensive leakage of lysosomal matrix enzymes. Electron microscopic observation revealed a remarkable aggregation of lysosomes by the basic polypeptides. Polyglutamic acid, an acidic polypeptide, did not produce such effect. ATP was found to stabilize lysosomes during incubation, particularly with basic polypeptides.