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Effect of polyethylene glycol on the activity, intrinsic fluorescence, and oligomeric structure of castor seed cytosolic fructose‐1, 6‐bisphosphatase
Author(s) -
R J Hodgson,
W C Plaxton
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00744-t
Subject(s) - fructose 1,6 bisphosphatase , chemistry , polyethylene glycol , fructose , peg ratio , enzyme , biochemistry , cytosol , size exclusion chromatography , chromatography , enzyme assay , fluorescence , physics , finance , quantum mechanics , economics
The effect of polyethylene glycol (PEG) on the activity, intrinsic fluorescence, and oligomeric structure of homogeneous cytosolic fructose‐1, 6‐bisphosphatase (FBPase c ) from endosperm of germinating castor oil seeds has been examined. Increasing the PEG concentration in the FBPase c reaction mixture elicited a progressive 3‐fold decrease in the enzyme'sK m for fructose‐1, 6‐P 2 . The presence of PEG also: (i) increased the extent of FBPase c inhibition by high levels of fructose‐1, 6‐P 2 , (ii) enhanced the intensity of the enzyme's fluorescence emission spectra, and (iii) prevents dissociation of the active tetrameric native enzyme into inactive lowerM r forms during gel filtration HPLC. It is concluded that the activity and structure of plant FBPase c is modified by extreme dilution, probably as a result of partial deaggregation of the native tetrameric enzyme.