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Human and other mammalian genomes contain transposons of the mariner family
Author(s) -
Corinne Augé-Gouillou,
Yves Bigot,
Nicolas Pollet,
MarieHélène Hamelin,
Michèle MeunierRotival,
Georges Périquet
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00735-r
Subject(s) - transposase , biology , genome , transposable element , genetics , gene , human genome , polymerase chain reaction , mobile genetic elements , dna , genomic dna , sequence (biology) , computational biology , microbiology and biotechnology
Internal fragments of the putative transposase gene of mariner ‐like elements (MLEs) were amplified from human, mouse, rat, chinese hamster, sheep and bovine genomic DNAs by polymerase chain reaction (PCR). The sequences identified in human, ovine and bovine genomes correspond to ancient degenerate transposons. Screening mammalian sequence libraries identified a truncated element in the human ABL gene and the sequence of its 5′‐ITR was determined. This ITR sequences were used in PCR experiments with DNA from six mammalian species and detected full‐sized and deleted MLEs. The presence of MLE in mammalian genomes demonstrates that they are ubiquitous mobile elements found from fungi to man. This observation strongly raises the possibility that MLE could constitute tools for the modification of eucaryotic genomes.