Premium
Characterization of the testis‐specific gene ‘calmegin’ promoter sequence and its activity defined by transgenic mouse experiments
Author(s) -
Dai Watanabe,
Masaru Okabe,
Naoki Hamajima,
Takashi Morita,
Yasuzo Nishina,
Yoshitake Nishimune
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00729-s
Subject(s) - biology , gene , microbiology and biotechnology , transgene , promoter , genomic dna , tata box , sequence (biology) , regulatory sequence , dna sequencing , dna , genetically modified mouse , sequence analysis , consensus sequence , genetics , gene expression , peptide sequence
We have cloned the genomic DNA of calmegin [(1992) J. Biol. Chem. 269, 7744–7749] and analyzed its promoter region. It contained GC‐rich sequences and potential binding sites for AP 2 and Sp 1, but lacked the TATA sequence. The 330 bp 5′ flanking sequence of calmegin genomic DNA fused with the CAT gene was used for the study of promoter activity in transgenic mice. The CAT gene activity was detected exclusively in testes, indicating that the 330 bp calmegin 5′ sequence was sufficient for the testis‐specific expression. The existence of testicular nuclear factors specifically bound to the putative promoter sequence was also demonstrated.