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Repression of transcriptional activity by heterologous KRAB domains present in zinc finger proteins
Author(s) -
Vissing H.,
Meyer W.K.-H.,
Aagaard L.,
Tommerup N.,
Thiesen H.-J.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00728-r
Subject(s) - zinc finger , krüppel , psychological repression , lim domain , genetics , biology , ring finger domain , phd finger , heterologous , protein domain , microbiology and biotechnology , gene , transcription factor , gene expression
We report the characterization of three novel members of the KRAB‐domain‐containing C 2 ‐H 2 zinc finger family (ZNF133, 136 and 140). KRAB ( Krüppel ‐associated box) is an evolutionarily conserved protein domain found N‐terminally with respect to the zinc finger repeats that encodes the DNA binding domain. ZNF133 and ZNF140 have both the KRAB A‐ and KRAB B‐boxes present at their N‐terminus, whereas ZNF136 contains only the KRAB A‐box. We have previously demonstrated that the KRAB domains derived from ZNF133 and ZNF140 are potent transcriptional repression domains [Margolin et al. (1994) Proc. Natl. Acad. Sci. USA 91, 4509–4513]. The KRAB domain from ZNF136, containing only subdomain A, is a considerable weaker suppression domain; however, when fused to the heterologous KRAB B subdomain of ZNF10 (KOX1) the two subdomains form a KRAB domain which induces repression as potently as previously reported KRAB domains.