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Novel actin crosslinker superfamily member identified by a two step degenerate PCR procedure
Author(s) -
Timothy J. Byers,
Alan H. Beggs,
Elizabeth M. McNally,
Louis M. Kunkel
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00722-l
Subject(s) - superfamily , actin , protein superfamily , spectrin , biology , primer (cosmetics) , ras superfamily , actin cytoskeleton , gene , cytoskeleton , genetics , microbiology and biotechnology , biochemistry , chemistry , enzyme , organic chemistry , cell , gtp'
Actin‐crosslinking proteins link F‐actin into the bundles and networks that constitute the cytoskeleton. Dystrophin, β‐spectrin, α‐actinin, ABP‐120, ABP‐280, and fimbrin share homologous actin‐binding domains and comprise an actin crosslinker superfamily. We have identified a novel member of this superfamily (ACF7) using a degenerate primer‐mediated PCR strategy that was optimized to resolve less‐abundant superfamily sequences. The ACF7 gene is on human chromosome 1 and hybridizes to high molecular weight bands on northern blots. Sequence comparisons argue that ACF7 does not fit into one of the existing families, but represents a new class within the superfamily.