Premium
Effects of fasting and refeeding on ob gene expression in white adipose tissue of lean and obese ( ob/ob ) mice
Author(s) -
Paul Trayhurn,
Margaret Thomas,
Jackie Duncan,
D V Rayner
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00719-p
Subject(s) - endocrinology , medicine , white adipose tissue , adipose tissue , refeeding syndrome , messenger rna , gene expression , biology , obesity , chemistry , gene , biochemistry , malnutrition
A 33‐mer antisense oligonucleotide has been utilized as a probe for the rapid chemiluminescence‐based detection of ob (obese) mRNA. Expression of the ob gene was evident in several white adipose tissue depots of mice (epididymal, highest; subcutaneous and omental, lowest), but not in other organs. Fasting (24 h) induced a substantial fall in ob mRNA in the epididymal fat of lean mice, which was rapidly reversed on refeeding, responses consistent with the putative role of ob in energy balance. Fasting had no effect, however, on ob mRNA levels in obese ( ob/ob ) mice.