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Molecular cloning of cDNA for sapecin B, an antibacterial protein of Sarcophaga , and its detection in larval brain
Author(s) -
Se Rok Lee,
Shoichiro Kurata,
Shunji Natori
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00717-n
Subject(s) - complementary dna , biology , cloning (programming) , microbiology and biotechnology , signal peptide , larva , cdna library , molecular cloning , biochemistry , peptide sequence , gene , computer science , programming language , botany
A cDNA clone for sapecin B, an antibacterial protein of Sarcophaga , was isolated. This cDNA encoded a precursor protein of sapecin B consisting of a signal sequence (24 residues), prosegment (30 residues) and mature sapecin B (34 residues). Sapecin B was synthesized almost exclusively in the fat body when the larval body wall was injured, but the brain of naive larvae was also demonstrated to contain a significant amount of sapecin B. These findings suggested that sapecin B is a bifunctional protein.