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Identification of two highly homologous presynaptic proteins distinctly localized at the dendritic and somatic synapses
Author(s) -
Seiichi Takahashi,
Hiroshi Yamamoto,
Zene Matsuda,
Michael Y. Ogawa,
Ken-ichi Yagyu,
Tadatsugu Taniguchi,
Takaki Miyata,
Hideto Kaba,
Takuma Higuchi,
Fumino Okutani
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00713-j
Subject(s) - somatic cell , homologous chromosome , biology , complementary dna , microbiology and biotechnology , cdna library , chemistry , gene , biochemistry
Through screening of a murine brain cDNA library, we have isolated two brain specific cDNAs encoding highly homologous proteins, named 921‐L and 921‐S, comprised of 134 amino acids with 80% identity. Immunohistological study with the mAbs raised against the bacterially expressed 921 proteins showed that 921‐L protein is distributed at the dendritic region and 921‐S at the neuronal somatic surface. Immuno‐electron microscopic study revealed that both 921 proteins are localized at the presynaptic terminal, indicating that the 921 proteins are differentially expressed at the dendritic and somatic presynapses.