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Sphingomyelin is synthesized at the plasma membrane of oligodendrocytes and by purified myelin membranes: a study with fluorescent‐ and radio‐labelled ceramide analogues
Author(s) -
Vos Jan P.,
Giudici M.Luisa,
van der Bijl Petra,
Magni Paola,
Marchesini Sergio,
van Golde Lambert M.G.,
Lopes-Cardozo Matthijs
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00695-6
Subject(s) - sphingomyelin , brefeldin a , ceramide , membrane , chemistry , golgi apparatus , microbiology and biotechnology , myelin , oligodendrocyte , biochemistry , cell , biology , central nervous system , endocrinology , apoptosis
In most cell types sphingomyelin is synthesized predominantly in the cis‐medial compartments of the Golgi stacks whereas the contribution of the plasma membrane is much lower. The aim of this study was to assess the contribution of both compartments to the synthesis of sphingomyelin in myelinating cells. Therefore, oligodendrocytes from rat spinal cord were incubated in culture with fluorescently‐ or radiolabelled ceramides, and the effects of a block in the vesicular flow (monensin, brefeldin A, low temperature) on surface synthesis of sphingomyelin were evaluated. The results indicate that ≈50% of the sphingomyelin synthase is present at the plasma and myelin membranes of oligodendrocytes.