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Evidence for SecA‐ and ΔpH‐independent insertion of D1 into thylakoids
Author(s) -
van Wijk Klaas Jan,
Knott Tracy G.,
Robinson Colin
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00668-y
Subject(s) - thylakoid , chloroplast , photosystem ii , azide , chemistry , biophysics , biochemistry , biology , photosynthesis , gene , organic chemistry
Many nuclear‐encoded proteins are targeted into chloroplast thylakoids by an azide sensitive Sec‐related mechanism or by a ΔpH‐driven mechanism. In this report, the requirements for the integration of chloroplast‐encoded thylakoid proteins have been analysed in pulse‐labeled intact chloroplasts. We show that the integration of the photosystem II reaction centre protein, D1, continues in the absence of a ΔpH and in the presence of azide. A range of other proteins are similarly targeted to thylakoids in the presence of azide, suggesting that the SecA‐related mechanism is not widely used for the targeting of chloroplast‐encoded proteins.