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Induction of the antioxidant stress proteins heme oxygenase‐1 and MSP23 by stress agents and oxidised LDL in cultured vascular smooth muscle cells
Author(s) -
Siow Richard C.M.,
Ishii Tetsuro,
Sato Hideyo,
Taketani Shigeru,
Leake David S.,
Sweiry Jacob H.,
Pearson Jeremy D.,
Bannai Shiro,
Mann Giovanni E.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00650-x
Subject(s) - heme oxygenase , oxidative stress , antioxidant , chemistry , heme , vascular smooth muscle , nadph oxidase , biochemistry , oxidative phosphorylation , endocrinology , biology , smooth muscle , enzyme
Enhanced expression of the antioxidant stress proteins heme oxygenase‐1 (HO‐1) and macrophage stress protein (MSP23) by oxidative stress agents and oxidatively modified low density lipoproteins (LDL) was investigated in cultured porcine aortic smooth muscle cells. Treatment of smooth muscle cells with glucose oxidase, CdCl 2 or diethylmaleate resulted in a time‐dependent (6–48 h) induction of HO‐1 and MSP23 expression. Exposure of cells to 100 μg protein/ml highly oxidised LDL increased the expression of HO‐1 and MSP23 within 24 h, and the induction was dependent on the degree of LDL oxidation. The induction of HO‐1 and MSP23 may thus play an important cytoprotective role against oxidative stress in atherogenesis.