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H 2 ‐forming N 5 , N 10 ‐methylenetetrahydromethanopterin dehydrogenase: mechanism of H 2 formation analyzed using hydrogen isotopes
Author(s) -
Klein Andreas R.,
Fernández Victor M.,
Thauer Rudolf K.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00642-m
Subject(s) - dehydrogenation , chemistry , hydrogen , isotope , crystallography , stereochemistry , physics , catalysis , organic chemistry , biochemistry , quantum mechanics
H 2 ‐forming N 5 , N 10 ‐methylenetetrahydromethanopterin dehydrogenase catalyzes the reversible dehydrogenation of N 5 , N 10 ‐methylenetetrahydromethanopterin (CH 2 = H 4 MPT) to N 5 , N 10 ‐methenyltetrahydromethanopterin (CH = H 4 MPT + ) and H 2 . In D 2 O both HD and D 2 are formed from CH 2 = H 4 MPT and in H 2 O both HD and H 2 from CD 2 = H 4 MPT. Evidence is presented that HD is not an intermediate in the formation of D 2 and H 2 , respectively.