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Stimulation of cell proliferation and autoregulation of elastin expression by elastin peptide VPGVG in cultured chick vascular smooth muscle cells
Author(s) -
Wachi Hiroshi,
Seyama Yoshiyuki,
Yamashita Sabrou,
Suganami Hideki,
Uemura Yuko,
Okamoto Kouji,
Yamada Haruyoshi,
Tajima Shingo
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00641-l
Subject(s) - elastin , collagenase , vascular smooth muscle , autoregulation , chemistry , cell growth , stimulation , microbiology and biotechnology , messenger rna , cell , biochemistry , smooth muscle , biology , endocrinology , enzyme , gene , genetics , blood pressure
Synthetic elastin peptides, VPGVG or its polymer (VPGVG) n , enhanced the proliferation of smooth muscle cells 1.5‐fold during 48 h treatment at the concetrations over 10 −6 M or 1.0 μg/ml, respectively. Monomeric and polymeric VPGVG sequences reduced elastin synthesis and its mRNA level to one‐third and one‐half of control respectively under the conditions in which the proliferation of cells were enhanced, but did not change collagen synthesis as measured by bacterial collagenase digestion. The elastin‐specific autoregulation by elastin fragments may reflect the feedback regulation of elastin expression which may play an essential role in elastin metabolism under the normal and diseased conditions.