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Insulin and insulin‐like growth factor I stimulate expression of the primary response gene cMG1/TIS11 b by a wortmannin‐sensitive pathway in RIE‐1 cells
Author(s) -
Corps Anthony N.,
Brown Kenneth D.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00635-m
Subject(s) - wortmannin , medicine , endocrinology , insulin , growth factor , phosphatidylinositol , insulin like growth factor , epidermal growth factor , insulin receptor , protein kinase c , insulin receptor substrate , biology , stimulation , chemistry , receptor , signal transduction , microbiology and biotechnology , insulin resistance
The addition of insulin or insulin‐like growth factor I (IGF‐I) to RIE‐1 cells increased the expression of the primary response gene cMG1; dose‐response analysis suggested that this effect was mediated largely through type 1 IGF receptors. Insulin/IGF‐I did not affect the expression of the cMG1‐related genes TIS11 and TIS11d, whereas epidermal growth factor, angiotensin II or 12‐ O ‐tetradecanoyl expression of all three genes. Incubation with wortmannin (WM) prevented the insulin/IGH‐I‐induced elevation of cMG1 mRNA, but not that induced by the other mitogens or the stimulation of mitogen‐activated protein kinase by insulin. We conclude that WM‐sensitive phosphatidylinositol 3‐kinase may be involved in the specific stimulation of cMG1 expression by insulin/IGF‐I.