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Simultaneous measurements of Ca 2+ in the intracellular stores and the cytosol of hepatocytes during hormone‐induced Ca 2+ oscillations
Author(s) -
Chatton Jean-Yves,
Liu Haiyan,
Stucki Jörg W.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00632-j
Subject(s) - cytosol , intracellular , thapsigargin , biophysics , chemistry , fura 2 , stimulation , endocrinology , biochemistry , biology , enzyme
Simultaneous Ca 2+ measurements in the cytosol and intracellular stores (IS) of rat hepatocytes were performed using two Ca 2+ ‐sensitive probes (Fluo‐3 and Mag‐fura‐2), and combined whole‐cell patch clamp and fluorescence microscopy. A steady‐state Ca 2+ concentration of ∼630 μM was estimated in the IS. α 1 ‐Adrenergic stimulation induced periodic elevations of cytosolic Ca 2+ and parallel synchronized transient declines in the IS. Subsequent application of the intracellular Ca 2+ ‐pump inhibitor thapsigargin resulted in a release of Ca 2+ from the IS to reach a level of Ca 2+ depletion much lower than the lowest transient decline observed during the oscillations.