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Mapping cells and sub‐cellular organelles on 2‐D gels: ‘new tricks for an old horse’
Author(s) -
Huber Lukas A.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00614-f
Subject(s) - organelle , horse , chemistry , microbiology and biotechnology , biophysics , biology , paleontology
Nowadays, investigators in all fields are faced with the identification of unknown, up‐ or down‐regulated, modified proteins that they are trying to identify. Two‐dimensional (2‐D) gel electrophoresis, with its ability to resolve several thousand proteins, is an extremely powerful technique. The current resolution and reproducibility of 2‐D gel technology and the establishment of computer assisted 2‐D gel protein databases have paved new ways for the identification of proteins.