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Long‐term enhancement of Na,K‐ATPase pump during blasttransformation of human lymphocytes is controlled first by translational, then by transcriptional mechanisms
Author(s) -
Marakhova I.I.,
Vereninov A.A.,
Toropova F.V.,
Vinogradova T.A.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00608-c
Subject(s) - cycloheximide , ouabain , translation (biology) , microbiology and biotechnology , biology , atpase , chemistry , cell , endogeny , protein biosynthesis , messenger rna , biochemistry , gene , enzyme , sodium , organic chemistry
The transition of phytohemagglutinin‐activated human lymphocytes from resting state to proliferation is accompanied by a long‐term increase in ouabain‐sensitive Rb(K) influx which is closely related to a cyclosporin A‐sensitive step of G0/G1/S progression. At least two distinct phases of the up‐regulation of cation pump has been revealed: the initial stage (5–20 h) which is cycloheximide‐inhibitable and actinomycin D (α‐amanitin)‐unaffected, and the later stage (after 20 h) which is cycloheximide‐ and actinomycin D (α‐amanitin)‐inhibitable. Thus, the enhanced Na,K‐ATPase pump during the cell progression from quiescence to proliferation is controlled both at translational and transcriptional levels.