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ID ‐ A dominant negative regulator of skeletal muscle differentiation — is not involved in maturation or differentiation of vascular smooth muscle cells
Author(s) -
Kemp Paul R.,
Metcalfe James C.,
Grainger David J.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00605-9
Subject(s) - vascular smooth muscle , myogenesis , myod , biology , regulator , medicine , skeletal muscle , endocrinology , myocyte , microbiology and biotechnology , gene expression , gene , biochemistry , smooth muscle
Rat aortic vascular smooth muscle cells (VSMCs) expressing the MyoD gene formed myotubes at a low frequency in the presence of serum but at a high frequency in the absence of serum. Expression of an antisense Id gene increased myotube formation in the presence of serum indicating that a reduction in Id levels is a major mechanism by which serum withdrawal promotes myotube formation. The role of Id in the development of VSMCs was investigated by expressing an antisense Id gene in neonatal VSMCs. No evidence was found for the conversion of neonatal VSMCs to adult VSMCs in the presence of the antisense Id gene. Similarly reduction in Id by serum withdrawal also failed to cause conversion of the neonatal VSMCs to the adult phenotype. These data suggest that the maturation of neonatal smooth muscle cells is not controlled by a VSMC homologue of the skeletal muscle basic‐helix‐loop‐helix proteins.