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Oxidative stress response in yeast: effect of glutathione on adaptation to hydrogen peroxide stress in Saccharomyces cerevisiae
Author(s) -
Izawa Shingo,
Inoue Yoshiharu,
Kimura Akira
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00603-7
Subject(s) - glutathione , saccharomyces cerevisiae , intracellular , biochemistry , oxidative stress , chemistry , hydrogen peroxide , glutathione synthetase , yeast , enzyme
Role of intracellular glutathione in the response of Saccharomyces cerevisiae to H 2 O 2 was investigated. Depletion of cellular glutathione or inhibition of γ‐glutamylcysteine synthetase (GSH‐I) enhanced the sensitivity to H 2 O 2 and suppressed the adaptation to H 2 O 2 . A mutant deficient in GSH‐I also showed the hypersensitivity and could not adapt to H 2 O 2 . Incubation of the cell with amino acids constituting glutathione ( l ‐Glu, l ‐Cys, Gly) increased the intracellular glutathione content, and subsequently the cell acquired resistance against H 2 O 2 . These results strongly suggest that intracellular glutathione plays an important role in the adaptive response in S. cerevisiae to oxidative damage.