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Inhibition of apoptosis by the expression of antisense Nedd2
Author(s) -
Kumar Sharad
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00602-6
Subject(s) - apoptosis , proteases , caenorhabditis elegans , programmed cell death , cytotoxic t cell , microbiology and biotechnology , biology , cell culture , caspase , serine protease , protease , chemistry , gene , enzyme , biochemistry , genetics , in vitro
Nedd2 belongs to a family of mammalian cysteine proteases which share similarity with the Caenorhabditis elegans cell death protein, Ced‐3. Overexpression of Nedd2 has been shown to induce apoptosis in mammalian cells but in the absence of a specific known inhibitor, it remains to be seen whether this represents cytotoxic effects of the Nedd2 protease or the specific activation of an apoptotic pathway. The present work shows that the factor‐dependent cell line FDC‐P1 expressing mouse antisense Nedd2 mRNA, exhibits significant inhibition of cell death upon removal of the cytokines, thus providing evidence for a direct role of Nedd2 in mediating apoptosis.