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A novel 6Fe (2 × [3Fe‐4S]) ferredoxin from Mycobacterium smegmatis
Author(s) -
Imai Takeo,
Urushiyama Akio,
Saito Hiroshi,
Sakamoto Yuki,
Ota Katsuya,
Ohmori Daijiro
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00601-5
Subject(s) - mycobacterium smegmatis , ferredoxin , chemistry , biochemistry , microbiology and biotechnology , biology , medicine , enzyme , mycobacterium tuberculosis , pathology , tuberculosis
A novel ferredoxin was purified from Mycobacterium smegmatis by a series of hydrophobic chromatographies in the presence of high concentrations of ammonium sulfate and sodium chloride. The ferredoxin exhibited the same peptide map and N‐terminal amino acid sequence as the known 7Fe ferredoxin from the same bacterium. On the other hand, this ferredoxin was found to contain ∼6 Fe/mol ferredoxin and was also shown to contain only [3Fe‐4S] clusters by resonance Raman spectroscopy, indicating that it is a novel 6Fe ferredoxin which contains two [3Fe‐4S] clusters.